Real‐time flow cytometry for the kinetic analysis of oncosis

  title={Real‐time flow cytometry for the kinetic analysis of oncosis},
  author={Gary Warnes and Sandra Martins},
  journal={Cytometry Part A},
The standard method of distinguishing apoptotic and oncotic cells has been by microscopic analysis of nuclei and cell membrane morphology. Thus a rapid test for analyzing large numbers of cells in the study of cell necrobiology has not been possible until the recent advent of the Amnis Image‐stream and real‐time Lab‐on‐a‐Chip technologies. An interesting difference between apoptosis and oncosis is that they are ATP dependent and independent processes, respectively. Here we describe an assay… 
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Methods for assessing mammalian cell death are presented and a discussion of the use of cytotoxicity assays to determine the mechanisms of cell death is discussed.
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  • Medicine
    Cytometry. Part A : the journal of the International Society for Analytical Cytology
  • 2012
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  • A. Tárnok
  • History, Medicine
    Cytometry. Part A : the journal of the International Society for Analytical Cytology
  • 2013
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