Reagentless identification of single bacterial spores in aqueous solution by confocal laser tweezers Raman spectroscopy.

@article{Chan2004ReagentlessIO,
  title={Reagentless identification of single bacterial spores in aqueous solution by confocal laser tweezers Raman spectroscopy.},
  author={J. W. M. Chan and Anthony P. Esp{\`o}sito and Chad E Talley and Christopher W Hollars and Stephen M. Lane and Thomas Huser},
  journal={Analytical chemistry},
  year={2004},
  volume={76 3},
  pages={599-603}
}
We demonstrate that optical trapping combined with confocal Raman spectroscopy using a single laser source is a powerful tool for the rapid identification of micrometer-sized particles in an aqueous environment. Optical trapping immobilizes the particle while maintaining it in the center of the laser beam path and within the laser focus, thus maximizing the collection of its Raman signals. The single particle is completely isolated from other particles and substrate surfaces, therefore… CONTINUE READING