Reading dynamic kinase activity in living cells for high-throughput screening.

@article{Allen2006ReadingDK,
  title={Reading dynamic kinase activity in living cells for high-throughput screening.},
  author={Michael D Allen and Lisa M. DiPilato and Meghdad Rahdar and Yunzhao R. Ren and Curtis Chong and Jun O Liu and Jin Zhang},
  journal={ACS chemical biology},
  year={2006},
  volume={1 6},
  pages={371-6}
}
Protein kinases, as crucial signaling molecules, represent an emerging class of drug targets, and the ability to assay their activities in living cells with high-throughput screening should provide exciting opportunities for drug discovery and chemical and functional genomics. Here, we describe a general method for high-throughput reading of dynamic kinase activities using ratiometric fluorescent sensors, and showcase an example of reading intracellular activities of protein kinase A (PKA) and… CONTINUE READING