Reactivity and biological importance of the disulfide bonds in human growth hormone

@article{Graf1976ReactivityAB,
  title={Reactivity and biological importance of the disulfide bonds in human growth hormone},
  author={László Gráf and J{\'a}nos Borvend{\'e}g and Erzs{\'e}bet Bar{\'a}t and Ilona Hermann and András Patthy},
  journal={FEBS Letters},
  year={1976},
  volume={66}
}
The role of human growth hormone's C-terminal disulfide bridge.
  • R. Junnila, Z. Wu, C. Strasburger
  • Biology, Medicine
    Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society
  • 2013
Significance of the disulphide bonds of human growth hormone.
TLDR
New data and old results concerning the disulphide bonds of GH are presented and relevant mutations found in patients with growth disorders are discussed, including those from patients carrying mutations in their GH1 gene.
Relationship between the antigenic topography and the structure of human growth hormone.
TLDR
It is concluded that the hGH structure defined by epitopes 3C11, 10C1, and HG3 is probably related to the binding properties of the hormone.
Short stature caused by a biologically inactive mutant growth hormone (GH-C53S).
TLDR
It is demonstrated that GH-C53S is a bioinactive GH at the physiological range and that the disulfide bridge Cys-53 toCys-163 is required for mediating the biological effects of GH.
Selective reduction of the disulfide bonds of ovine placental lactogen.
Reduction and carbamidomethylation of two of the three disulfide bridges of ovine placental lactogen was accomplished by the use of 20-fold molar excess of dithiothreitol over protein disulfide
Preclinical characterization of eleven new Cys-PEGylated hGH mutants
Short stature explained by dimerization of human growth hormone induced by a p.C53S point mutation
TLDR
The unpaired Cys-165 in GH-C53S forms a disulfide bond linking two hGH molecules in pituitary cells, which is responsible for the growth failure in the affected individual.
Characterisation of a trisulphide derivative of biosynthetic human growth hormone produced in Escherichia coli.
A novel protein derivative has been found during process development of biosynthetic human growth hormone; it has been characterised as human growth hormone with a Cys182-Cys189 trisulphide bridge.
New insights into the biological activity and secretion properties of a polypeptide derived from tilapia somatotropin.
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References

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TLDR
The chemistry involved in the sulfitolysis of growth hormone was sufficiently interesting to extend the study to include porcine and human GH, and the hope that it would be active in primates has not been realized.
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TLDR
The ability of dithiothreitol to effect the reduction of the disulfide bonds in five proteins under mild conditions and in the absence of denaturant has been examined and Lysozyme, prolactin, and insulin appeared to be quantitatively reduced.
Retention of the Biological Potency of Human Pituitary Growth Hormone After Reduction and Carbamidomethylation
TLDR
The reduced alkylated derivative of human pituitary growth hormone was prepared by reacting the native hormone in 8M urea solution, first with mercaptoethanol and then with iodoacetamide, and it appears that the full potency of thenative hormone is retained in the reduced alKYlated derivative.
Human pituitary growth hormone: isolation and properties of two biologically active fragments from plasmin digests.
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TLDR
Two biologically active fragments have been isolated from plasmic digests of human pituitary growth hormone and were shown to be derived from the cleavage of the Arg-Thr and Lys-Gln bonds of the hormone.
Bioassay of hypophyseal growth hormone; the tibia test.
TLDR
This paper attempts to standardize the procedure and apply it to the assay of pure and crystalline growth hormone, finding that the method is highly sensitive and simple as compared to other techniques.
Bio-assay of prolactin. Analysis of the pigeon crop-sac response to local prolactin injection by an objective and quantitative method.
TLDR
Mucosal dry weight responses were highly correlated with response diameter and response area measurements, and the responsiveness of the crop-sac ME to local prolactin injection was unrelated to either the initial thick...
DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS *
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The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Endocrinology 45,455-463
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