Reactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymes

  title={Reactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymes},
  author={David S Hewings and Johanna Heideker and Taylur P. Ma and Andrew P AhYoung and Farid El Oualid and Alessia Amore and Gregory T. Costakes and Daniel Kirchhofer and Bradley Brasher and Thomas H Pillow and Nataliya Popovych and Till Maurer and Carsten Schwerdtfeger and William F. Forrest and Kebing Yu and John A. Flygare and Matthew S Bogyo and Ingrid E. Wertz},
  booktitle={Nature Communications},
Activity-based probes (ABPs) are widely used to monitor the activity of enzyme families in biological systems. Inferring enzyme activity from probe reactivity requires that the probe reacts with the enzyme at its active site; however, probe-labeling sites are rarely verified. Here we present an enhanced chemoproteomic approach to evaluate the activity and probe reactivity of deubiquitinase enzymes, using bioorthogonally tagged ABPs and a sequential on-bead digestion protocol to enhance the… CONTINUE READING
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