Reactive oxygen species and cryopreservation promote DNA fragmentation in equine spermatozoa.

@article{Baumber2003ReactiveOS,
  title={Reactive oxygen species and cryopreservation promote DNA fragmentation in equine spermatozoa.},
  author={Julie Baumber and Barry A Ball and Jennifer J Linfor and Stuart A. Meyers},
  journal={Journal of andrology},
  year={2003},
  volume={24 4},
  pages={621-8}
}
The objective of this study was to examine the effect of reactive oxygen species (ROS) and cryopreservation on DNA fragmentation of equine spermatozoa. In experiment 1, equine spermatozoa were incubated (1 hour, 38 degrees C) according to the following treatments: 1) sperm alone; 2) sperm + xanthine (X, 0.3 mM)-xanthine oxidase (XO, 0.025 U/mL); 3) sperm + X (0.6 mM)-XO (0.05 U/mL); and 4) sperm + X (1 mM)-XO (0.1 U/mL). In experiment 2, spermatozoa were incubated (1 hour, 38 degrees C) with X… CONTINUE READING

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