Reactivation of the hydrogenase from Desulfovibrio gigas by hydrogen. Influence of redox potential.

Abstract

The specific activity of the periplasmic hydrogenase from Desulfovibrio gigas is increased approximately 10-fold in the H2 utilization assay with benzyl viologen by several hours of incubation under an atmosphere of H2. After a variable lag phase during which residual traces of O2 are removed, the reversible activation is exponential. The extent of activation is dependent on pH and the redox potential of the incubation medium. A tentative model based on the existence of a monoelectronic redox center is proposed as shown in the following equation: (formula; see text) The potential of this redox couple was determined to be -310 mV (pH = 7; T = 298 K) versus the normal hydrogen electrode.

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@article{Lissolo1984ReactivationOT, title={Reactivation of the hydrogenase from Desulfovibrio gigas by hydrogen. Influence of redox potential.}, author={Thierry Lissolo and Sylviane Pulvin and Daniel W. Thomas}, journal={The Journal of biological chemistry}, year={1984}, volume={259 19}, pages={11725-9} }