Reactivation of dormant "non-culturable" Mycobacterium tuberculosis developed in vitro after injection in mice: both the dormancy depth and host genetics influence the outcome.

@article{Shleeva2015ReactivationOD,
  title={Reactivation of dormant "non-culturable" Mycobacterium tuberculosis developed in vitro after injection in mice: both the dormancy depth and host genetics influence the outcome.},
  author={M. Shleeva and T. Kondratieva and E. Rubakova and G. Vostroknutova and A. Kaprelyants and A. Apt},
  journal={Microbial pathogenesis},
  year={2015},
  volume={78},
  pages={
          63-6
        }
}
Three stocks of Mycobacterium tuberculosis H37Rv were cultured in vitro under prolonged hypoxic or acidified conditions until partial or complete loss of the capacity to form colonies on agar medium was achieved. Such dormant "non-culturable" mycobacteria were assessed for the growth resuscitation after intra-tracheal injection into mice of the two inbred strains with different genetic susceptibility to M. tuberculosis-triggered disease: hyper-susceptible I/St and relatively resistant B6. The… Expand
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