Reactivation of a glycerinated model of Amoeba

Abstract

Motile models ofAmoeba proteus prepared by extraction at −20 °C with a 50% buffered glycerol solution showed remarkable contraction on addition of Mg-ATP with retainment of Ca++ sensitivity. The initial contraction around the nucleus occurred on addition of Mg-ATP independently of the Ca++ concentration, and was followed by contractions of three different patterns. In 10−8M Ca++, the granuloplasm contracted as a whole and separated from the membrane leaving no detectable particles in the anterior region. In 10−6M Ca++, the granuloplasm contracted leaving some granules which showed active and vigorous two-directional streaming similar to that in the living cell. Sometimes a part of the plasma membrane twitched. The streaming lasted up to 20 minutes. In 10−4M Ca++, after the initial contraction around the nucleus, no significant movement was observed. All these movements in the models were inhibited by cytochalasin B or N-ethylmaleimide. The relationship between the Mg-ATP and the Ca++ concentrations was examined.

DOI: 10.1007/BF01287635

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@article{Kuroda2005ReactivationOA, title={Reactivation of a glycerinated model of Amoeba}, author={Kiyoko Kuroda and Seiji Sonobe}, journal={Protoplasma}, year={2005}, volume={109}, pages={127-142} }