Reactions of phosphorothioate compounds catalyzed by adenylosuccinate synthetase. Steady state and pre-steady state kinetic studies.

Abstract

Kinetic studies of the reactions catalyzed by adenylosuccinate synthetase with phosphorothioate derivatives of substrates for the forward and reverse reactions provide evidence for an intermediate on the reaction pathway. Guanosine-5’-0-(3-thiotriphosphate) (GTPyS) replaces GTP with a 12.5-fold reduction in the maximal velocity (at 22”C), although the K… (More)

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