Reactions of fluorescent probes with normal and chemically modified myelin.

@article{Feinstein1975ReactionsOF,
  title={Reactions of fluorescent probes with normal and chemically modified myelin.},
  author={Maurice B. Feinstein and H W Felsenfeld},
  journal={Biochemistry},
  year={1975},
  volume={14 14},
  pages={3041-8}
}
The fluorescent probes 8-anilino-1-naphthalenesulfonate (ANS) and 2-p-toluidinylnaphthalene-6-sulfonate (TNS) bind to highly purified myelin membranes obtained from bovine brain white matter. Binding of the dyes was markedly increased by environmental conditions which reduce the negative surface potential of the membrane, i.e., cations (La-3+ is greater than Ca-2+ is greater than Na-+,K-+), H-+, local anesthetics, and the antibiotic polymyxin B. Chemical alteration of accessible membrane… CONTINUE READING