Reactions at the polymerase active site that contribute to the fidelity of Escherichia coli DNA polymerase I (Klenow fragment).

@article{Joyce1992ReactionsAT,
  title={Reactions at the polymerase active site that contribute to the fidelity of Escherichia coli DNA polymerase I (Klenow fragment).},
  author={Catherine M. Joyce and Xiaojun Sun and Nigel D. F. Grindley},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 34},
  pages={24485-500}
}
In order to study the structural principles governing DNA polymerase fidelity we have measured the rates of insertion of incorrect nucleotides and the rates of extension from the resulting mismatched base pairs, catalyzed by the Klenow fragment of DNA polymerase I. Using a combination of semi-quantitative and qualitative approaches, we have studied each of the 12 possible mismatches in a variety of sequence contexts. The results indicate that Klenow fragment discriminates between mismatches… CONTINUE READING