Reaction of ovine prolactin with 2-(2-nitrophenylsulfenyl)-3-methyl-3'-bromoindolemine.

  title={Reaction of ovine prolactin with 2-(2-nitrophenylsulfenyl)-3-methyl-3'-bromoindolemine.},
  author={Richard A. Houghten and Choh hao Li},
  journal={International journal of peptide and protein research},
  volume={11 5},
The two tryptophan bonds at positions 91 and 150 in ovine prolactin have been cleaved by the use of 2-(2-nitrophenylsulfenyl)-3-methyl-3'-bromoindolemine. The reaction yielded three products: a small fragment containing residues 92-150, a large fragment consisting of residues 1-91 and 151-199 joined by a disulfide bond, and a derivative containing uncleaved but oxidized prolactin. The yield of the two fragments was 38% and 26%, respectively, indicating a cleavage of approximately 55% for each… CONTINUE READING