Reaction of ferric cytochrome P450cam with peracids: kinetic characterization of intermediates on the reaction pathway.

@article{Spolitak2005ReactionOF,
  title={Reaction of ferric cytochrome P450cam with peracids: kinetic characterization of intermediates on the reaction pathway.},
  author={Tatyana Spolitak and John H. Dawson and David P. Ballou},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 21},
  pages={20300-9}
}
Reactions of substrate-free ferric cytochrome P450cam with peracids to generate Fe=O intermediates have previously been investigated with contradictory results. Using stopped-flow spectrophotometry, the reaction with m-chloroperoxybenzoic acid demonstrated an Fe(IV)=O + porphyrin pi-cation radical (Cpd I) (Egawa, T., Shimada, H., and Ishimura, Y. (1994) Biochem. Biophys. Res. Commun. 201, 1464-1469). By contrast, with peracetic acid, Fe(IV)=O plus a tyrosyl radical were observed by freeze… CONTINUE READING
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Referenced Papers

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Showing 1-3 of 3 references

Heme Peroxidases, Willey-VCH, New York

  • H. B. Dunford
  • 1999

Cytochrome P450: Structure, Mechanism and Biochemistry, 2nd ed

  • P. Ortiz de Montellano
  • 1995

244-248. by gest on O cber 1, 2017 hp://w w w .jb.org/ D

  • G. C. Wagner, M. M. Palcic, H. B. Dunford
  • FEBS Lett
  • 1983

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