Reaction of ferric cytochrome P450cam with peracids: kinetic characterization of intermediates on the reaction pathway.

@article{Spolitak2005ReactionOF,
  title={Reaction of ferric cytochrome P450cam with peracids: kinetic characterization of intermediates on the reaction pathway.},
  author={Tatyana Spolitak and John H. Dawson and David P. Ballou},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 21},
  pages={20300-9}
}
Reactions of substrate-free ferric cytochrome P450cam with peracids to generate Fe=O intermediates have previously been investigated with contradictory results. Using stopped-flow spectrophotometry, the reaction with m-chloroperoxybenzoic acid demonstrated an Fe(IV)=O + porphyrin pi-cation radical (Cpd I) (Egawa, T., Shimada, H., and Ishimura, Y. (1994) Biochem. Biophys. Res. Commun. 201, 1464-1469). By contrast, with peracetic acid, Fe(IV)=O plus a tyrosyl radical were observed by freeze… CONTINUE READING