Reaction of DNA with chemically or enzymatically activated mitomycin C: isolation and structure of the major covalent adduct.

@article{Tomasz1986ReactionOD,
  title={Reaction of DNA with chemically or enzymatically activated mitomycin C: isolation and structure of the major covalent adduct.},
  author={Maria Tomasz and Dondapati Chowdary and Ruth Lipman and Sakurako Shimotakahara and D Veiro and Valerie Walker and Gregory L. Verdine},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1986},
  volume={83 18},
  pages={6702-6}
}
The antitumor antibiotic mitomycin C is shown to form a covalent complex with calf thymus DNA under anaerobic conditions in the presence of either NADPH cytochrome c reductase/NADPH, xanthine oxidase/NADH, or the chemical reducing system H2/PtO2. Digestion of the complex with DNase I/snake venom diesterase/alkaline phosphatase yields a single mitomycin deoxyguanosine adduct as the major DNA alkylation product, identified as N2-(2'' beta,7''-diaminomitosen-1'' alpha-yl) 2'-deoxyguanosine… CONTINUE READING
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Antibiotics 1: Mechanism of Action

  • W. Szybalski, V. N. Iyer
  • 1967

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