Reaction mechanism of non-allosteric phosphofructokinase.

  title={Reaction mechanism of non-allosteric phosphofructokinase.},
  author={Wilhelm Simon and Hans Werner Hofer},
  journal={European journal of biochemistry},
  volume={88 1},
The reaction mechanism of the non-allosteric phosphofructokinase from Lactobacillus plantarum was investigated by initial-rate bisubstrate kinetics and product inhibition kinetics adn by the measurement of equilibrium isotope exchange in the presence of various substrate and product concentrations. The reaction mechanism is clearly sequential. The product inhibition and equilibrium isotope-exchange patterns are consistent with an ordered bi-bi reaction sequence with fructose 6-phosphate as the… CONTINUE READING

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