Reaction intermediate partitioning by ribulose-bisphosphate carboxylases with differing substrate specificities.

@article{Pierce1986ReactionIP,
  title={Reaction intermediate partitioning by ribulose-bisphosphate carboxylases with differing substrate specificities.},
  author={J. Pierce and T. Andrews and G. Lorimer},
  journal={The Journal of biological chemistry},
  year={1986},
  volume={261 22},
  pages={
          10248-56
        }
}
The carboxylated, 6-carbon reaction intermediate (3-keto-2-carboxyarabinitol 1,5-bisphosphate) from the ribulose-1,5-bisphosphate carboxylase reaction was obtained by denaturing the enzyme with acid during steady-state turnover. Carbon-13 NMR analysis indicates that this beta-keto acid exists in solution predominantly as the C-3 ketone (as opposed to the hydrate) form. In neutral solution the intermediate slowly decomposes (t1/2 approximately 1 h) by decarboxylation. This decarboxylation… Expand
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