Reactibodies generated by kinetic selection couple chemical reactivity with favorable protein dynamics.

@article{Smirnov2011ReactibodiesGB,
  title={Reactibodies generated by kinetic selection couple chemical reactivity with favorable protein dynamics.},
  author={Ivan V. Smirnov and Eug{\'e}nie Carletti and Inna N. Kurkova and Florian Nachon and Yvain Nicolet and Vladimir A Mitkevich and H{\'e}l{\`e}ne D{\'e}bat and B{\'e}rang{\`e}re Avalle and Alexey Belogurov and Nikita Kuznetsov and Andrey V Reshetnyak and Patrick Masson and Alexander G. Tonevitsky and Natalia Ponomarenko and Alexander A. Makarov and Alain Friboulet and Alfonso Tramontano and Alexandre G. Gabibov},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2011},
  volume={108 38},
  pages={
          15954-9
        }
}
Igs offer a versatile template for combinatorial and rational design approaches to the de novo creation of catalytically active proteins. We have used a covalent capture selection strategy to identify biocatalysts from within a human semisynthetic antibody variable fragment library that uses a nucleophilic mechanism. Specific phosphonylation at a single tyrosine within the variable light-chain framework was confirmed in a recombinant IgG construct. High-resolution crystallographic structures of… CONTINUE READING
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