Re-evaluation of the 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) assay for antioxidant activity.

@article{Xie2014ReevaluationOT,
  title={Re-evaluation of the 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) assay for antioxidant activity.},
  author={Jie Fen Xie and Karen M Schaich},
  journal={Journal of agricultural and food chemistry},
  year={2014},
  volume={62 19},
  pages={4251-60}
}
Kinetics and stoichiometry of reactions between the 2,2-diphenyl-1-picrylhydrazyl (DPPH) stable radical and 25 antioxidant compounds with different structure, molecular weight, number of -OH groups, and redox potential were investigated by recording the loss of DPPH(•) absorbance at 515 nm continuously for 10 min. A series of antioxidant concentrations was tested to determine linear response ranges and reaction saturation points. The primary feature distinguishing antioxidant activity--rate of… CONTINUE READING
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