Ray Wu, fifth business or father of DNA sequencing?

@article{Xue2016RayWF,
  title={Ray Wu, fifth business or father of DNA sequencing?},
  author={Yu Xue and Yongbo Wang and Hui Shen},
  journal={Protein \& Cell},
  year={2016},
  volume={7},
  pages={467 - 470}
}
The first author, Yu Xue, first read about Dr. Wu in the ScienceNet blog written in 2008 by Prof. Yi Rao, an eminent Chinese neurobiologist, who has made a profound and lasting influence on the new generation of Chinese scientists, including us. Later, this essay was included in one of his published books, and we carefully read it again during the Spring Festival of 2015. One sentence was quite confusing to us, “The primer-extension approach developed by Ray Wu (Fig. 1) in 1971, is a key step… 
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References

SHOWING 1-10 OF 22 REFERENCES
For Our founding father of ABRC, Prof. Ray Wu (1928–2008)
  • N. Yang, C. Lan
  • Biology
    Science in China Series C: Life Sciences
  • 2009
TLDR
Ray Wu was a pioneer in DNA sequencing and recombinant DNA technology, a renowned scientist in rice genetic engineering, a strong and long time advocate for scientific research and education in Chinese Taipei and Chinese Mainland, and most of all, a genuine mentor and true friend to many of us, worldwide and at ABRC.
Ray Wu as Fifth Business: Deconstructing collective memory in the history of DNA sequencing.
  • Lisa Onaga
  • Biology
    Studies in history and philosophy of biological and biomedical sciences
  • 2014
Nucleotide sequence analysis of DNA.
  • R. Wu
  • Biology, Chemistry
    Nature: New biology
  • 1972
TLDR
By incorporating highly labelled deoxynucleotides into DNA in vitro, small amounts of material can be used for sequence analysis and the nucleotide sequence of DNA molecules can now be determined from the 5′-terminal.
Nucleotide sequence analysis of DNA. IX. Use of oligonucleotides of defined sequence as primers in DNA sequence analysis.
  • R. Padmanabhan, R. Wu
  • Biology, Chemistry
    Biochemical and biophysical research communications
  • 1972
Nucleotide sequence analysis of DNA. IV. Complete nucleotide sequence of the left-hand cohesive end of coliphage 186 DNA.
Nucleotide sequence analysis of DNA. I. Partial sequence of the cohesive ends of bacteriophage lambda and 186 DNA.
  • R. Wu
  • Biology, Chemistry
    Journal of molecular biology
  • 1970
Structure and base sequence in the cohesive ends of bacteriophage lambda DNA.
Nucleotide sequence analysis of deoxyribonucleic acid. VI. Determination of 3'-terminal dnucleotide sequences of several species of duplex deoxyribonucleic acid using Escherichia coli deoxyribonucleic acid polymerase I.
TLDR
Under the conditions studied the initial rate of polymerization is at least three times faster than the rate of interchange using 186 DNA, a DNA substrate in which both reactions can be measured simultaneously.
DNA sequencing with chain-terminating inhibitors.
TLDR
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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