Rationally reduced libraries for combinatorial pathway optimization minimizing experimental effort.

@article{Jeschek2016RationallyRL,
  title={Rationally reduced libraries for combinatorial pathway optimization minimizing experimental effort.},
  author={Markus Jeschek and Daniel Gerngross and Sven Panke},
  journal={Nature communications},
  year={2016},
  volume={7},
  pages={11163}
}
Rational flux design in metabolic engineering approaches remains difficult since important pathway information is frequently not available. Therefore empirical methods are applied that randomly change absolute and relative pathway enzyme levels and subsequently screen for variants with improved performance. However, screening is often limited on the… CONTINUE READING