Rationally designed short polyisoprenol-linked PglB substrates for engineered polypeptide and protein N-glycosylation.

@article{Liu2014RationallyDS,
  title={Rationally designed short polyisoprenol-linked PglB substrates for engineered polypeptide and protein N-glycosylation.},
  author={Feng Liu and Balakumar Vijayakrishnan and Amirreza Faridmoayer and Thomas A. Taylor and Thomas B. Parsons and Gonçalo J L Bernardes and Michael Kowarik and Benjamin G Davis},
  journal={Journal of the American Chemical Society},
  year={2014},
  volume={136 2},
  pages={566-9}
}
The lipid carrier specificity of the protein N-glycosylation enzyme C. jejuni PglB was tested using a logical, synthetic array of natural and unnatural C10, C20, C30, and C40 polyisoprenol sugar pyrophosphates, including those bearing repeating cis-prenyl units. Unusual, short, synthetically accessible C20 prenols (nerylnerol 1d and geranylnerol 1e) were shown to be effective lipid carriers for PglB sugar substrates. Kinetic analyses for PglB revealed clear K(M)-only modulation with lipid chain… CONTINUE READING
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