Rationally designed short polyisoprenol-linked PglB substrates for engineered polypeptide and protein N-glycosylation.

@article{Liu2014RationallyDS,
  title={Rationally designed short polyisoprenol-linked PglB substrates for engineered polypeptide and protein N-glycosylation.},
  author={Feng Liu and Balakumar Vijayakrishnan and Amirreza Faridmoayer and Thomas A. Taylor and Thomas B. Parsons and Gonçalo J L Bernardes and Michael Kowarik and Benjamin G Davis},
  journal={Journal of the American Chemical Society},
  year={2014},
  volume={136 2},
  pages={566-9}
}
The lipid carrier specificity of the protein N-glycosylation enzyme C. jejuni PglB was tested using a logical, synthetic array of natural and unnatural C10, C20, C30, and C40 polyisoprenol sugar pyrophosphates, including those bearing repeating cis-prenyl units. Unusual, short, synthetically accessible C20 prenols (nerylnerol 1d and geranylnerol 1e) were… CONTINUE READING