Rationally designed fluorogenic protease reporter visualizes spatiotemporal dynamics of apoptosis in vivo.

@article{To2015RationallyDF,
  title={Rationally designed fluorogenic protease reporter visualizes spatiotemporal dynamics of apoptosis in vivo.},
  author={Tsz-Leung To and Beverly J. Piggott and Kalpana Makhijani and Dan Yu and Yuh Nung Jan and Xiaokun Shu},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2015},
  volume={112 11},
  pages={3338-43}
}
Fluorescence resonance energy transfer-based reporters have been widely used in imaging cell signaling; however, their in vivo application has been handicapped because of poor signal. Although fluorogenic reporters overcome this problem, no such reporter of proteases has been demonstrated for in vivo imaging. Now we have redesigned an infrared fluorescent protein so that its chromophore incorporation is regulated by protease activity. Upon protease activation, the infrared fluorogenic protease… CONTINUE READING

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A survey of 6,300 genomic fragments for cis-regulatory activity in the imaginal discs of Drosophila melanogaster

  • A Jory
  • Cell Reports 2(4):1014–1024
  • 2012

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