Rationalizing molecular analysis of field-collected roots for assessing diversity of arbuscular mycorrhizal fungi: to pool, or not to pool, that is the question

@article{Renker2006RationalizingMA,
  title={Rationalizing molecular analysis of field-collected roots for assessing diversity of arbuscular mycorrhizal fungi: to pool, or not to pool, that is the question},
  author={Carsten Renker and Karoline Weisshuhn and Harald Kellner and François Buscot},
  journal={Mycorrhiza},
  year={2006},
  volume={16},
  pages={525-531}
}
For rationalizing molecular analysis of field-collected roots in diversity studies on arbuscular mycorrhiza, we compared three different approaches. After DNA extraction from 50 root samples of Plantago lanceolata grown on monoculture plots at a former arable field site, (1) DNAs were amplified separately by nested PCR and each amplicon was cloned separately; (2) DNAs were amplified separately by nested PCR, 1 μl of each amplicon was pooled, and a single cloning was made from the resulting… CONTINUE READING
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BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT

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