Rational stabilization of the C-LytA affinity tag by protein engineering.

@article{HernndezRocamora2008RationalSO,
  title={Rational stabilization of the C-LytA affinity tag by protein engineering.},
  author={V{\'i}ctor M. Hern{\'a}ndez-Rocamora and Beatriz Maestro and Almudena Moll{\'a}-Morales and Jes{\'u}s M Sanz},
  journal={Protein engineering, design & selection : PEDS},
  year={2008},
  volume={21 12},
  pages={709-20}
}
The C-LytA protein constitutes the choline-binding module of the LytA amidase from Streptococcus pneumoniae. Owing to its affinity for choline and analogs, it is regularly used as an affinity tag for the purification of proteins in a single chromatographic step. In an attempt to build a robust variant against thermal denaturation, we have engineered several… CONTINUE READING