Rational engineering of human cytochrome P450 2B6 for enhanced expression and stability: importance of a Leu264->Phe substitution.

@article{Kumar2007RationalEO,
  title={Rational engineering of human cytochrome P450 2B6 for enhanced expression and stability: importance of a Leu264->Phe substitution.},
  author={Santosh Kumar and Yonghong Zhao and Ling Min Sun and Surendra S. Negi and James R. Halpert and Bilikallahalli K. Muralidhara},
  journal={Molecular pharmacology},
  year={2007},
  volume={72 5},
  pages={
          1191-9
        }
}
Despite the emerging importance of human P450 2B6 in xenobiotic metabolism, thorough biochemical and biophysical characterization has been impeded as a result of low expression in Escherichia coli. Comparison with similar N-terminal truncated and C-terminal His-tagged constructs (rat P450 2B1dH, rabbit 2B4dH, and dog 2B11dH) revealed that P450 2B6dH showed the lowest thermal stability, catalytic tolerance to temperature, and chemical stability against guanidinium chloride-induced denaturation… CONTINUE READING

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