Rational engineering of cytochromes P450 2B6 and 2B11 for enhanced stability: Insights into structural importance of residue 334.

@article{Talakad2010RationalEO,
  title={Rational engineering of cytochromes P450 2B6 and 2B11 for enhanced stability: Insights into structural importance of residue 334.},
  author={Jyothi C. Talakad and P Ross Wilderman and D. R. Davydov and Santosh Kumar and James R. Halpert},
  journal={Archives of biochemistry and biophysics},
  year={2010},
  volume={494 2},
  pages={
          151-8
        }
}
Rational mutagenesis was used to improve the thermal stability of human cytochrome P450 2B6 and canine P450 2B11. Comparison of the amino acid sequences revealed seven sites that are conserved between the stable 2B1 and 2B4 but different from those found in the less stable 2B6 and 2B11. P334S was the only mutant that showed increased heterologous expression levels and thermal stability in both 2B6 and 2B11. The mechanism of this effect was explored with pressure-perturbation spectroscopy… CONTINUE READING
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