Rational design of fluorescein-based fluorescence probes. Mechanism-based design of a maximum fluorescence probe for singlet oxygen.

@article{Tanaka2001RationalDO,
  title={Rational design of fluorescein-based fluorescence probes. Mechanism-based design of a maximum fluorescence probe for singlet oxygen.},
  author={Keiji Tanaka and Tomoya Miura and Naoki Umezawa and Yasuteru Urano and Kazuya Kikuchi and Tsunehiko Higuchi and Takayuki Nagano},
  journal={Journal of the American Chemical Society},
  year={2001},
  volume={123 11},
  pages={2530-6}
}
Fluorescein is one of the best available fluorophores for biological applications, but the factors that control its fluorescence properties are not fully established. Thus, we initiated a study aimed at providing a strategy for rational design of functional fluorescence probes bearing fluorescein structure. We have synthesized various kinds of fluorescein derivatives and examined the relationship between their fluorescence properties and the highest occupied molecular orbital (HOMO) levels of… CONTINUE READING