Rational design of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 to increase alpha-cyclodextrin production.

  title={Rational design of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 to increase alpha-cyclodextrin production.},
  author={Bart A. van der Veen and Joost C. M. Uitdehaag and Dirk Penninga and G J van Alebeek and Landon M. Smith and Bauke W. Dijkstra and Lubbert Dijkhuizen},
  journal={Journal of molecular biology},
  volume={296 4},
Cyclodextrin glycosyltransferases (CGTase) (EC are extracellular bacterial enzymes that generate cyclodextrins from starch. All known CGTases produce mixtures of alpha, beta, and gamma-cyclodextrins. A maltononaose inhibitor bound to the active site of the CGTase from Bacillus circulans strain 251 revealed sugar binding subsites, distant from the catalytic residues, which have been proposed to be involved in the cyclodextrin size specificity of these enzymes. To probe the importance… 

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