Rational design of an orthogonal tryptophanyl nonsense suppressor tRNA

  title={Rational design of an orthogonal tryptophanyl nonsense suppressor tRNA},
  author={Randall A. Hughes and Andrew D. Ellington},
  booktitle={Nucleic acids research},
While a number of aminoacyl tRNA synthetase (aaRS):tRNA pairs have been engineered to alter or expand the genetic code, only the Methanococcus jannaschii tyrosyl tRNA synthetase and tRNA have been used extensively in bacteria, limiting the types and numbers of unnatural amino acids that can be utilized at any one time to expand the genetic code. In order to expand the number and type of aaRS/tRNA pairs available for engineering bacterial genetic codes, we have developed an orthogonal… CONTINUE READING


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A new orthogonal suppressor tRNA/Aminoacyl-tRNA synthetase pair for evolving an organism with an expanded genetic code

  • M. Pastrnak, T. M. Mageliery, P. G. Schultz
  • Helv. Chim. Acta,
  • 2000
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