Ratiometric imaging of calcium during ischemia-reperfusion injury in isolated mouse hearts using Fura-2

@inproceedings{Venkataraman2012RatiometricIO,
  title={Ratiometric imaging of calcium during ischemia-reperfusion injury in isolated mouse hearts using Fura-2},
  author={Raghav Venkataraman and Mark R Holcomb and Rene Harder and Bj{\"o}rn C Knollmann and Franz J. Baudenbacher},
  booktitle={Biomedical engineering online},
  year={2012}
}
BACKGROUND We present an easily implementable method for measuring Fura-2 fluorescence from isolated mouse hearts using a commercially available switching light source and CCD camera. After calibration, it provides a good estimate of intracellular [Ca2+] with both high spatial and temporal resolutions, permitting study of changes in dispersion of diastolic [Ca2+], Ca2+ transient dynamics, and conduction velocities in mouse hearts. In a proof-of-principle study, we imaged isolated Langendorff… CONTINUE READING

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