Ratiometric Sensing and Imaging of Intracellular pH Using Polyethylenimine-Coated Photon Upconversion Nanoprobes.

@article{Nreoja2017RatiometricSA,
  title={Ratiometric Sensing and Imaging of Intracellular pH Using Polyethylenimine-Coated Photon Upconversion Nanoprobes.},
  author={Tuomas N{\"a}reoja and Takahiro Deguchi and Simon Christ and Riikka Peltomaa and Neeraj Prabhakar and Elnaz Fazeli and Niina Per{\"a}l{\"a} and Jessica M Rosenholm and Riikka Arppe and Tero Soukka and Michael Schaeferling},
  journal={Analytical chemistry},
  year={2017},
  volume={89 3},
  pages={1501-1508}
}
Measurement of changes of pH at various intracellular compartments has potential to solve questions concerning the processing of endocytosed material, regulation of the acidification process, and also acidification of vesicles destined for exocytosis. To monitor these events, the nanosized optical pH probes need to provide ratiometric signals in the optically transparent biological window, target to all relevant intracellular compartments, and to facilitate imaging at subcellular resolution… CONTINUE READING