Ratio-based decisions and the quantitative analysis of cDNA microarray images.

@article{Chen1997RatiobasedDA,
  title={Ratio-based decisions and the quantitative analysis of cDNA microarray images.},
  author={Yuhchyau Chen and Edward R. Dougherty and Michael L. Bittner},
  journal={Journal of biomedical optics},
  year={1997},
  volume={2 4},
  pages={
          364-74
        }
}
Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA micro-array. Comparison of gene expression levels arising from co-hybridized samples is achieved by taking ratios of average expression levels for individual genes. In an image-processing phase, a method of image segmentation identifies cDNA target sites in a cDNA micro-array image. The resulting cDNA target sites are analyzed based on a hypothesis test and confidence interval to quantify the… CONTINUE READING

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