Rates of Chemical Reactions Embedded in a Metabolic Network by Dissolution Dynamic Nuclear Polarisation NMR.

@article{Sadet2018RatesOC,
  title={Rates of Chemical Reactions Embedded in a Metabolic Network by Dissolution Dynamic Nuclear Polarisation NMR.},
  author={Aude Sadet and Emmanuelle M M Weber and Aditya Jhajharia and Dennis Kurzbach and Geoffrey Bodenhausen and Emeric Miclet and Daniel Abergel},
  journal={Chemistry},
  year={2018},
  volume={24 21},
  pages={5456-5461}
}
The isomerisation of 6-phosphogluconolactones and their hydrolyses into 6-phosphogluconic acid form a non enzymatic side cycle of the pentose-phosphate pathway (PPP) in cells. Dissolution dynamic nuclear polarisation can be used for determining the kinetic rates of the involved transformations in real time. It is found that the hydrolysis of both lactones is significantly slower than the isomerisation process, thereby shedding new light onto this subtle chemical process.