Rate-limiting steps in activation of cardiac Cl- current revealed by photolytic application of cAMP.

@article{Nakashima1994RatelimitingSI,
  title={Rate-limiting steps in activation of cardiac Cl- current revealed by photolytic application of cAMP.},
  author={Yoshiyuki Nakashima and Katsushige Ono},
  journal={The American journal of physiology},
  year={1994},
  volume={267 4 Pt 2},
  pages={
          H1514-22
        }
}
Single myocytes were obtained from guinea-pig ventricles, and the catecholamine-induced Cl- current (ICl) was recorded by the patch-clamp method combined with the use of caged adenosine 3',5'-cyclic monophosphate (cAMP) and the concentration jump technique. The rapid bath application of isoprenaline was followed by a marked latency of approximately 1.7 s before a sigmoidal onset of the ICl activation, while the photolysis of caged cAMP was followed by no measurable latency. The ICl conductance… CONTINUE READING
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