Rat ventral mesencephalon grown as organotypic slice cultures and co-cultured with striatum, hippocampus, and cerebellum

@article{stergaard1990RatVM,
  title={Rat ventral mesencephalon grown as organotypic slice cultures and co-cultured with striatum, hippocampus, and cerebellum},
  author={Karen \Ostergaard and J. P. Schou and Jens Zimmer},
  journal={Experimental Brain Research},
  year={1990},
  volume={82},
  pages={547-565}
}
Tissue slices of rat ventral mesencephalon (VM), striatum, hippocampus and cerebellum were prepared from late fetal (E21) to 7 day old (P7) rats and cultured for 3 to 60 days by the roller tube technique before they were stained immunocytochemically for tyrosine hydroxylase (TH), a marker of dopaminergic (DA) neurons and fibres. The TH immunoreactive (TH-i), DA neurons retained their morphological in vivo characteristics in the VM slice cultures consisting of the substantia nigra (SN) and the… CONTINUE READING

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