Rat liver thioredoxin and thioredoxin reductase: purification and characterization.

@article{Luthman1982RatLT,
  title={Rat liver thioredoxin and thioredoxin reductase: purification and characterization.},
  author={Marguerite Luthman and Anne Holmgren},
  journal={Biochemistry},
  year={1982},
  volume={21 26},
  pages={
          6628-33
        }
}
A reproducible scheme has been developed for the preparation of rat liver thioredoxin and thioredoxin reductase (EC 1.6.4.5) by using assays based on reduction of insulin and 5,5'-dithiobis(2-nitrobenzoic acid), respectively. Both proteins were purified to homogeneity, as judged from polyacrylamide gel electrophoresis. Thioredoxin had a molecular weight of 12 000 and contained about 110 amino acids including 4 half-cystines and an NH2-terminal valine. Peptide maps of reduced and… 
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31 References

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