Rat kidney glomerular basement membrane visualized in situ by embedment-free sectioning and subsequent platinum-carbon replication.

  • Hideaki Kondo
  • Published 1990 in Journal of electron microscopy technique

Abstract

Following the removal of polyethylene glycol (PEG) from thin sections, and viewing through the endothelial fenestrae and/or the interpedicel spaces, the rat renal glomerular basement membrane in situ was revealed to consist of meshworks and to be electron-transparent when examined at right angles to the plane of the membrane. By subsequent platinum replication of the embedment-free sections, the lamina densa of the basement membrane appeared as a veil composed of rather closely packed particles. The architecture of the split diaphragm and the surface morphology of the endothelial cell membrane were also clearly revealed. The present results indicate that the PEG method, with or without replication, can provide valuable information on basement membrane morphology.

Cite this paper

@article{Kondo1990RatKG, title={Rat kidney glomerular basement membrane visualized in situ by embedment-free sectioning and subsequent platinum-carbon replication.}, author={Hideaki Kondo}, journal={Journal of electron microscopy technique}, year={1990}, volume={14 1}, pages={63-9} }