Rat hepatic glutaminase: purification and immunochemical characterization.

  title={Rat hepatic glutaminase: purification and immunochemical characterization.},
  author={Erik M. Smith and Malcolm Watford},
  journal={Archives of biochemistry and biophysics},
  volume={260 2},
A method for the purification of phosphate-activated glutaminase from the liver of streptozotocin-diabetic rats is described. The procedure involves solubilization of glutaminase activity from isolated mitochondria by sonication, followed by ammonium sulfate precipitation, polyethylene glycol precipitation, and sequential chromatography on DEAE, hydroxylapatite, and zinc-chelated resins. The enzyme was purified 600-fold to a specific activity of 31-57 U/mg protein. The purified enzyme has an… CONTINUE READING