Rat embryonic motoneurons in long-term co-culture with Schwann cells--a system to investigate motoneuron diseases on a cellular level in vitro.

@article{Haastert2005RatEM,
  title={Rat embryonic motoneurons in long-term co-culture with Schwann cells--a system to investigate motoneuron diseases on a cellular level in vitro.},
  author={Kirsten Haastert and Julian Grosskreutz and Martin Jaeckel and Christina Laderer and Johannes Bufler and Claudia B. Grothe and Peter Claus},
  journal={Journal of neuroscience methods},
  year={2005},
  volume={142 2},
  pages={275-84}
}
Investigations of motoneuron diseases on a cellular and molecular level require long-term cultivation of primary cells. Here we present a new culture system in which matured motoneurons interact with their physiological partners like interneurons, astroglia and peripheral glia cells. This enables motoneuron-maturation for up to 3 weeks, while motoneurons consistently reached large diameters of their somata of 30-45 microm, occasionally more than 80 microm. Dissociated rat embryonic ventral… CONTINUE READING

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