Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: its potential role in the association between alcohol drinking and breast tumor promotion.

@article{Castro2003RatBM,
  title={Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: its potential role in the association between alcohol drinking and breast tumor promotion.},
  author={Gerardo Daniel Castro and Aurora M A Delgado de Lay{\~n}o and Mart{\'i}n Hern{\'a}n Costantini and Jos{\'e} Alberto de Castro},
  journal={Teratogenesis, carcinogenesis, and mutagenesis},
  year={2003},
  volume={Suppl 1},
  pages={61-70}
}
We recently showed that mammary cytosolic xanthineoxidoreductase had the ability to bioactivate ethanol (EtOH) to acetaldehyde (AC) and free radicals. In the present study, we report that the microsomal fraction also biotransforms EtOH to AC. One pathway requires NADPH and the others do not. Both need oxygen. The NADPH-dependent pathway is not inhibited by CO:O(2) (80:20) or SKF 525A and that excludes the participation of cytochrome P450. It is inhibited by diethyldithiocarbamate (DDTC), sodium… CONTINUE READING