Rat bone marrow-derived mast cells co-cultured with 3T3 fibroblasts in the absence of T-cell derived cytokines require stem cell factor for their survival and maintain their mucosal mast cell-like phenotype.

@article{MacDonald1996RatBM,
  title={Rat bone marrow-derived mast cells co-cultured with 3T3 fibroblasts in the absence of T-cell derived cytokines require stem cell factor for their survival and maintain their mucosal mast cell-like phenotype.},
  author={Alisdair J. MacDonald and Elizabeth M. Thornton and George F.J. Newlands and Stephen J Galli and Redwan Moqbel and Hugh R. P. Miller},
  journal={Immunology},
  year={1996},
  volume={88 3},
  pages={375-83}
}
When cultured without fibroblasts, rat bone marrow-derived mast cells (BMMC) contain abundant rat mast cell proteinase type II (RMCP-II), and exhibit survival and proliferation when maintained in mesenteric lymph node conditioned medium (CM). When BMMC were co-cultured with 3T3 fibroblasts in the absence of CM, BMMC numbers increased for 7 days and the BMMC… CONTINUE READING