Rapidly cooled human sperm: no evidence of intracellular ice formation.

@article{Morris2006RapidlyCH,
  title={Rapidly cooled human sperm: no evidence of intracellular ice formation.},
  author={George John Morris},
  journal={Human reproduction},
  year={2006},
  volume={21 8},
  pages={2075-83}
}
BACKGROUND The cellular damage that human spermatozoa encounter at rapid rates of cooling has often been attributed to the formation of intracellular ice. However, no direct evidence of intracellular ice has been presented. Alternatively, the cell damage may be the result of an osmotic imbalance encountered during thawing. This article examines whether intracellular ice forms during rapid cooling or if an alternative mechanism is present. METHODS In this study, human spermatozoa were cooled… CONTINUE READING