Rapid whole-genome sequencing for investigation of a neonatal MRSA outbreak.

  title={Rapid whole-genome sequencing for investigation of a neonatal MRSA outbreak.},
  author={Claudio U K{\"o}ser and Matthew T. G. Holden and Matthew J. Ellington and Edward J. Cartwright and Nicholas M. Brown and Amanda L Ogilvy-Stuart and Li Yang Hsu and Claire Chewapreecha and Nicholas J. Croucher and Simon R. Harris and Mandy Sanders and Mark C. Enright and Gordon Dougan and Stephen D. Bentley and Julian Parkhill and Louise J. Fraser and Jason R Betley and Ole Schulz-Trieglaff and Geoffrey P. Smith and Sharon J Peacock},
  journal={The New England journal of medicine},
  volume={366 24},
BACKGROUND Isolates of methicillin-resistant Staphylococcus aureus (MRSA) belonging to a single lineage are often indistinguishable by means of current typing techniques. Whole-genome sequencing may provide improved resolution to define transmission pathways and characterize outbreaks. METHODS We investigated a putative MRSA outbreak in a neonatal intensive care unit. By using rapid high-throughput sequencing technology with a clinically relevant turnaround time, we retrospectively sequenced… CONTINUE READING