Rapid visual detection of sperm-egg fusion using the DNA-specific fluorochrome Hoechst 33342.

@article{Hinkley1986RapidVD,
  title={Rapid visual detection of sperm-egg fusion using the DNA-specific fluorochrome Hoechst 33342.},
  author={Robert E. Hinkley and Bonnie D Wright and J W Lynn},
  journal={Developmental biology},
  year={1986},
  volume={118 1},
  pages={148-54}
}
When unfertilized sea urchin eggs are pretreated with the bisbenzimide DNA-specific fluorochrome Hoechst 33342, then washed and fertilized, a single sperm bound to the egg surface becomes intensely fluorescent. The location of the fluorescent sperm on the egg surface coincides exactly with the epicenter of the cortical reaction and the site at which the insemination cone subsequently appears. These observations, coupled with studies of eggs treated with quercetin to prevent fusion, as well as… CONTINUE READING

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