Rapid vertical tube rotor gradient assay for binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin to the Ah receptor.

The Ah receptor, which regulates induction of aryl hydrocarbon hydroxylase (cytochrome P1-450), can be assayed using [3H] 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as the radioligand. Sucrose density gradient (SDG) centrifugation is the most reliable method for detecting and quantitating Ah receptor; however, previous gradient assays in swinging bucket… CONTINUE READING