Rapid ultraperformance liquid chromatography-tandem mass spectrometry assay for a characteristic glycogen-derived tetrasaccharide in Pompe disease and other glycogen storage diseases.

@article{Sluiter2012RapidUL,
  title={Rapid ultraperformance liquid chromatography-tandem mass spectrometry assay for a characteristic glycogen-derived tetrasaccharide in Pompe disease and other glycogen storage diseases.},
  author={Wim J. Sluiter and Jeroen C van den Bosch and Daphne A Goudriaan and Carin M van Gelder and Juna M. de Vries and Jan G. M. Huijmans and Arnold J. J. Reuser and Ans T van der Ploeg and George J. G. Ruijter},
  journal={Clinical chemistry},
  year={2012},
  volume={58 7},
  pages={1139-47}
}
BACKGROUND Urinary excretion of the tetrasaccharide 6-α-D-glucopyranosyl-maltotriose (Glc₄) is increased in various clinical conditions associated with increased turnover or storage of glycogen, making Glc₄ a potential biomarker for glycogen storage diseases (GSD). We developed an ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) assay to detect Glc₄ in urine without interference of the Glc₄ isomer maltotetraose (M₄). METHODS Urine samples, diluted in 0.1% ammonium… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 3 times over the past 90 days. VIEW TWEETS