Rapid two-step procedure for large-scale purification of pediocin-like bacteriocins and other cationic antimicrobial peptides from complex culture medium.

Abstract

A rapid and simple two-step procedure suitable for both small- and large-scale purification of pediocin-like bacteriocins and other cationic peptides has been developed. In the first step, the bacterial culture was applied directly on a cation-exchange column (1-ml cation exchanger per 100-ml cell culture). Bacteria and anionic compounds passed through the… (More)

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@article{Uteng2002RapidTP, title={Rapid two-step procedure for large-scale purification of pediocin-like bacteriocins and other cationic antimicrobial peptides from complex culture medium.}, author={Marianne Uteng and H{\aa}vard Hildeng Hauge and Ilia Brondz and Jon Nissen-Meyer and Gunnar Fimland}, journal={Applied and environmental microbiology}, year={2002}, volume={68 2}, pages={952-6} }