Rapid two-dimensional immunoelectrophoresis of human serum proteins.

  title={Rapid two-dimensional immunoelectrophoresis of human serum proteins.},
  author={George L. Wright and Lee. Pollack and D. B. Roberts},
  journal={Clinical chemistry},
  volume={20 1},
We describe two versions of a two-dimensional immunoelectrophoretic system and illustrate their use for separating and estimating human serum proteins. Each is sensitive, rapid, and simple to perform, and requires no special apparatus and only 0.1-0.6 ml of antiserum. Both procedures appear to be potentially useful in the clinical laboratory. Time required to obtain a stained pattern varies from 4.5 to 7 h, depending on the intended purpose and sensitivity (number of possible precipitin peaks… 

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